incubar

Los microcosmos se incubaron durante 45 días en condiciones controladas.	Microcosms were incubated for 45 days under controlled conditions.
Se añadió PEDV y las células se incubaron durante 4 horas.	PEDV was added and cells were incubated for 4 hours.
Las plantas se incubaron a 22°C y a 95% de humedad.	Plants were incubated at 22°C and 95% humidity.
Las placas se incubaron a 37oC durante 24 horas en condiciones de anaerobiosis.	Plates were incubated at 37oC for 24 hours under anaerobic conditions.
Todos los viales se incubaron a temperatura ambiente (25oC) en condiciones de anaerobiosis.	All vials were incubated at room temperature (25oC) under anaerobic conditions.
Después de la transfección, las células se incubaron durante 48 h antes de realizar otras pruebas experimentales.	After transfection, cells were incubated for another 48 h before being subjected to other experimental assays.
En otros experimentos se incubaron nonaBDE (BDE-207 y BDE-206), que se degradaron a mezclas de octaBDE.	In separate experiments, the nona-BDEs BDE-207 and BDE-206 were incubated and were degraded to mixtures of octa-BDEs.
El licor ruminal y el agropiro se incubaron a 39 °C en saliva artificial preparada con agua de bebida.	Rumen fluid and tall wheatgrass were incubated at 39 °C in artificial saliva prepared with drinking water.
Luego las semillas se incubaron a temperaturas constantes (25 y 30 oC) y a temperaturas variables (20-30 oC).	The seeds were then incubated at a constant temperature (25 or 30 oC) and alternate temperatures (20-30 oC).
Etapa 29 (E6) explantes de retina enteros se incubaron con la Cdk1 / 2 Inhibidor III durante 4 horas y se analizó la inmunorreactividad PH3.	Stage 29 (E6) whole retinal explants were incubated with the Cdk1/2 Inhibitor III for 4 hr and PH3 immunoreactivity was analyzed.
Se bloquearon las placas con albúmina de suero bovino al 5% y se incubaron con una dilución de 1:100 de suero humano por duplicado.	Plates were blocked with 5% bovine serum albumin and were incubated with a 1:100 dilution of human serum in duplicate.
Materiales y métodos: las células se sembraron en placas de 96 pozos y se incubaron con sucralosa (Splenda®) por 60 minutos, stevia (Svetia®) o metformina.	Methods: Cells were cultured in 96-well ELISA plates, then incubated with sucralose, stevia or metformin.
Las muestras se incubaron a 37 o C durante 20-24 horas en una atmósfera con 2-5% de CO2 (método de jarra con vela).	Specimens were incubated at 37 o C for 20 to 24 hours in an atmosphere containing 2% to 5% CO2 (candle jar method).
Se recubrieron placas de microtitulación con 0,5 μg del antígeno específico en tampón de carbonato-bicarbonato 0,05 M, a pH 9,6, y se incubaron (durante una noche) a 4 °C.	Microtiter plates were coated with 0.5 μg of the specific antigen in 0.05 M carbonate-bicarbonate buffer, pH 9.6 and were incubated overnight at 4 °C.
Se observó actividad sinérgica antirretrovírica cuando se incubaron linfocitos T humanos infectados con la variante LAI del VIH-1 con indinavir y con zidovudina, didanosina o INNTIs.	Synergistic antiretroviral activity was observed when human T– lymphoid cells infected with the LAI variant of HIV– 1 were incubated with indinavir and either zidovudine, didanosine, or NNRTIs.
A continuación, los cortes se incubaron en una solución de cloruro de 2,3,5-trifeniltetrazolio al 2% durante 20 min a 37 °C. Por último, se examinaron bajo luz ambiental y se fotografiaron.	Thereafter, slices were incubated in 2,3,5-triphenyltetrazolium chloride 2% solution for 20 min at 37 o C. Finally, they were viewed under room light and photographed.
Las muestras se desinfestaron con hipoclorito de sodio, se colocaron en cámaras con alta humedad relativa e incubaron a 25 ± 2 °C en oscuridad durante 30 días, para el desarrollo de estructuras reproductivas.	Samples were disinfected with sodium hypochlorite, placed in chambers with high relative humidity and incubated at 25 ± 2 °C in darkness during 30 days for the development of reproductive structures.
Las muestras se incubaron en tampón fosfato o SHSE (37 ° C / pH = 7,0) durante hasta 180 días, luego se suspendieron en un fermentador de biofilm de flujo continuo que cultivaba biofilms de Streptococcus mutans UA159.	Specimens were incubated in phosphate-buffer or SHSE (37 °C/pH = 7.0) for up to 180 days, then suspended in a continuous flow biofilm fermenter cultivating biofilms of Streptococcus mutans UA159.
De forma simultánea, se realizó un control negativo en el que se usó agua peptonada tamponada (BPW), contaminada con la misma concentración de microorganismos que incubaron en las mismas condiciones para establecer la diferencia de crecimiento.	Simultaneously a negative control using buffered penton water (BPW) was contaminated with the same concentration of microorganisms and incubated under the same conditions to determine difference in growth.
Para cada muestra, se incubaron 100 μ g de proteína total durante 5 min a 95 °C en tampón de muestra Laemmli, y se separaron mediante electroforesis en condiciones reductoras en gel de poliacrilamida al 12%.	For each sample, 100 μg of total protein were incubated for 5 minutes at 95oC in Laemmli sample buffer and were separated by means of electrophoresis under reductive conditions in 12% polyacrylamide gel.